Forensics

Multiplex PCR

Multiplex PCR is a widespread molecular biology technique widely used in Short Tandem Repeat and Single Nucleotide Polymorphism analysis. Multiplex PCR is a polymerase chain reaction in which multiple DNA templates can be amplified by using multiple primer pairs in a single reaction. The method was first described in 1988 to detect deletions in dystrophin …

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Quantitative PCR

Quantitative PCR  (Real-time PCR) is a molecular biology technique for the amplification of targeted DNA molecules in real-time. Real-time PCR is a recently developed technique by Higuchi in 1993. This procedure uses a modified thermal cycler with UV Detector and Charged couple device (CCD) camera. The procedure measures the amount of amplicon produced in each …

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DNA Extraction

DNA extraction/isolation is the purification of DNA using combination of various methods. DNA Extraction is a significant procedure in molecular biological methods and in forensic analysis. Several different extraction kits and protocols/methods are available. Proper extraction methods and procedures should be chosen to get the process optimized and also to save time. Basic procedures for …

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