Saranya Krishnan

Hi, I am Saranya. I hold MS Forensic Science (Biology track) from International Forensic Research Institute (IFRI),Florida International University, Miami and MSc Biotechnology from Bharatiar University, India. During my graduate degree in US, myresearch work was in the Genomic Analyses of pathogenic and Non pathogenic Fungi isolated from ambrosia beetles in avocado trees using Fragment analysis and Multi locus sequence typing. I am a person passionate in Forensic Biology, especially the role of DNA in Forensics.

What is DNA Extraction?

DNA extraction/isolation is the purification of DNA using combination of various methods. DNA Extraction is a significant procedure in molecular biological methods and in forensic analysis. Several different extraction kits and protocols/methods are available. Proper extraction methods and procedures should be chosen to get the process optimized and also to save time. Basic procedures for …

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ANALYSIS OF SALIVARY ENZYME IN HUMAN

Saliva is an extracellular fluid secreted by salivary glands in the mouth. Saliva in humans, consists of water, electrolytes, mucus, white blood cells, epithelial cells, amylase, lipase, IgA, and lysozymes. Alpha amylase is present in humans, elephants, rats, and pigs. Amylase in humans is 42-59 U/ml. STARCH IODINE PRESUMPTIVE TEST Enzyme amylase is a component …

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SEMEN Analysis

Semen, also known as Spermatozoa is a type of body fluid that is secreted by sexual organs of male reproductive system in order to fertilize female ova. In certain cases to analyze rape or sexual assaults, presumptive and confirmatory test of semen is performed. Screening test is performed to analyze semen. Semen can be visualized …

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SEROLOGY

What is Forensic Serology? Forensic serologist  analyze semen, feces, urine, saliva etc. Serology is the identification of blood and blood proteins. Serology is  described as a range of laboratory tests utilizing antigen and serum antibody reactions. There are two different types of blood/body fluids transfer. Direct transfer and indirect transfer. Current serological tests analyses  the …

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Sanger Sequencing

Sequencing is a process of determining the precise order of nucleotides in a DNA template after amplification using specific loci. Sanger sequencing was invented by Fredrick Sanger in 1977.It is a widely used sequencing method and now replaced by Next-Generation sequencing methods. In Sanger sequencing, only  fragments of DNA (specific locus) can be amplified and …

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Length Heterogeneity Polymerase Chain Reaction

Length Heterogeneity Polymerase Chain Reaction (LH-PCR) reveals the length heterogeneity of amplicons. The procedure indicates the natural variations of insertion and deletion of nucleotides. LH PCR exposes the allelic profile pattern of DNA in a particular loci. Species-specific differentiation is possible using this method. The procedure is rapid simple and reproducible.  LH-PCR/Fragment Analysis is an …

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