Saranya Krishnan

Hi, I am Saranya. I hold MS Forensic Science (Biology track) from International Forensic Research Institute (IFRI),Florida International University, Miami and MSc Biotechnology from Bharatiar University, India. During my graduate degree in US, myresearch work was in the Genomic Analyses of pathogenic and Non pathogenic Fungi isolated from ambrosia beetles in avocado trees using Fragment analysis and Multi locus sequence typing. I am a person passionate in Forensic Biology, especially the role of DNA in Forensics.

Multiplex PCR

Multiplex PCR is a widespread molecular biology technique widely used in Short Tandem Repeat and Single Nucleotide Polymorphism analysis. Multiplex PCR is a polymerase chain reaction in which multiple DNA templates can be amplified by using multiple primer pairs in a single reaction. The method was first described in 1988 to detect deletions in dystrophin …

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Quantitative PCR

Quantitative PCR  (Real-time PCR) is a molecular biology technique for the amplification of targeted DNA molecules in real-time. Real-time PCR is a recently developed technique by Higuchi in 1993. This procedure uses a modified thermal cycler with UV Detector and Charged couple device (CCD) camera. The procedure measures the amount of amplicon produced in each …

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DNA Extraction

DNA extraction/isolation is the purification of DNA using combination of various methods. DNA Extraction is a significant procedure in molecular biological methods and in forensic analysis. Several different extraction kits and protocols/methods are available. Proper extraction methods and procedures should be chosen to get the process optimized and also to save time. Basic procedures for …

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